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Objective To investigate the incidence of thyroid-stimulating hormone and its correlation with other biochemical markers of blood glucose and blood fat in health examination population.Methods Physical examination results of 1 070 staff from the First Affiliated Hospital of Hebei North University from January to May 2017,including serum thyroid-stimulating hormone(TSH),total cholesterol (CHOL),triglyceride (TG),and glucose (GLU),H bA 1 c were sorted,grouped by gender and age,and retrospectively analyzed.Results A total of 118 cases of TSH positive were detected,,the positive rate was 11.03 ％,of which 58 were male,the positive rate was 9.40％ and 60 were women,the positive rate was 13.25％,there was a statistically significant difference in the positive rate of TSH between two groups of male and female (x2=9.819,P＜ 0.05).TSH was positively related to TG,LDI,and GLU (r=0.006,0.048,0.021;P=0.038,0.017,0.045),and is negatively related to HDL(r=-0.017,P=0.024),and had no relation with CHOL and HbA1c(P＞ 0.05).There was a significant difference in the level of CHOL between the TSH group and the normal TSH groups (P＜0.05).Conclusion TSH has become a major factor affecting human health and should be highly valued.
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Objective To observe the effects of 630 nm red light and 460 nm blue light emitting diode irradiation on the healing of skin wounds in Japanese big-ear white rabbits. Methods The skin wound model was established with 8 Japanese big-ear white rabbits. Three parts of vulnus in each rabbit were used:two parts of vulnus were irradiated vertically by red and blue LED light,respectively(15 min/time),and the distance between lights and wounds was 15 cm;the 3part of the wound was used as a control. On the 21day of the wounds exposure to light,the number of healing wounds and the percentage of healing area were recorded and the treatment effect of these two light sources was compared. HE staining was used to analyze the newborn tissue structure. Masson staining was used to observe the proliferation of skin collagen fibers. Immuohistochemical staining was used to analyze fibroblast growth factor(FGF),epidermal growth factor(EGF),endothelial growth factor(CD31),proliferating cell nuclear antigen(Ki-67),and inflammatory cytokines(CD68)infiltration in the skin. Results The healing rate in the red light,blue light,and control groups was 50.0%(4/8),25.0%(2/8),and 12.5%(1/8),respectively. Since the 12day after modeling,the healing area percentage in the red light group was significantly higher than those in the blue light and control groups(P<0.05,P<0.01). On the 21day after modeling,the skin thickness of the red light group was(2.95±0.34)mm,which was significantly higher than that in control group [(2.52±0.42)mm;F=3.182,P=0.016)]. The average optical density of collagen fibers was 0.15±0.03 in red light group,which was significantly higher than that of the blue light group(0.09±0.01;F=7.316,P=0.012)and control(0.07±0.01;F=7.316,P=0.003). The results of immunohistochemistry showed the expression levels of EGF,FGF,CD31 antigen,and Ki-67 in the red light group were significantly higher than those in the blue light and control groups,whereas the CD68 expression was significantly lower(P<0.05 or P<0.01). Conclusion LED red light irradiation can promote the healing of skin wounds in Japanese big-ear white rabbits,which may be achieved by the effect of red light irradiation in stimulating the proliferation of skin epidermal cells,vascular endothelial cells,and fiberous tissue.
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Objective To evaluate the efficacy of Ganoderma lucidum preparation on the behaviors,biochemistry,and autoimmunity parameters of mouse models of APP/PS-1 double transgenic Alzheimer's disease(AD).Methods A total of 44 4-month-old APP/PS-1 double transgenic AD mice were randomly divided into AD model group,Aricept group,Ganoderma lucidum middle-dose(LZ-M)group,and Ganoderma lucidum high-dose(LZ-H)group,with 11 mice in each group.In addition,10 4-month-old C57BL/6 mice were used as the control group.Water maze test was conducted to observe the behavior changes,and the protein expressions in brain tissues were detected by Western blot analysis.The autoimmune indicators were detected by indirect immunofluorescence method.Results In the navigation experiment,the time of finding the platform was gradually shortened since the 2day in the control,LZ-H,and LZ-M groups,and the time of searching the platform in the AD model group gradually increased.On the 5day,the time of finding platform was significantly shorter in control group (t=5.607,P=0.000) and LZ-H group(t=2.750,P=0.010)than AD model group.In the space exploration experiment,the number of crossing the target platform(t=2.452,P=0.025)and the residence time in the target quadrant(t=2.530,P=0.020)in AD model group mice was significantly smaller/shorter than those in control group;in addition,the number of crossing the target platform in the AD model group was significantly smaller than that in LZ-H group(t=2.317,P=0.030)and LZ-M group(t=2.443,P=0.030),while the residence time in target quadrant decreased significantly(t=2.770,P=0.020)compared with LZ-H group;the number of crossing through the target platform quadrant(t=2.493,P=0.022)and residence time in the target quadrant(t=2.683,P=0.015)in LZ-H group were significantly higher than in Aricept group.Western blot analysis showed that the expression of ApoA1 in the brain tissues of mice in LZ-H and LZ-M groups were significantly higher than those in AD model group(P<0.01,P<0.05);Aβ-40 expression in LZ-H group was significantly lower than that in AD model group(P<0.05);the expressions of Syt1,ApoE,and ABCA1 in brain tissues of mice in LZ-H group were significantly higher than those in model group(P<0.01,P<0.05).The plasma IgG level in Aricept group(t=30.945,P=0.000),LZ-M group(t=25.639,P=0.000)and LZ-H group(t=4.689,P=0.001)were significantly higher than that in the control group.Conclusion Ganoderma lucidum preparation can improve behavior disorders of AD model mice,promote the expressions of ApoA1,ApoE and Syt1,inhibit the expression of Aβ-40 protein,and improve the autoimmune function.
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Objective To investigate the spontaneous neoplastic lesions and their incidences in rats .Methods Sixty male and 60 female specific pathogen-free Wistar rats (4-weeks old ) were used in this study .The rats were acclima-ted for 1 week prior to initiation of the experiment .They were fed with conventional feed for 104 weeks and then sacrificed for histopathological examination .Results Various neoplastic lesions of the rats and their incidences were analyzed and reported.For male rats, their total tumor incidence was 49.12%, the benign tumor incidence was 38.60%and the malig-nant tumor incidence was 17.54%.The benign neoplastic lesions mainly were pituitary adenoma ( 19.30%) , testis Leydig cell tumor (5.26%) and subcutaneous fibroma (5.26%).The malignant neoplastic lesions mainly were squamous cell carcinoma (7.02%) and lymphoid hematopoietic system tumors (3.51%).For female rats, their total tumor inci-dence was 60.34%, the benign tumor incidence was 50.00%and the malignant tumor incidence was 15.52%.The benign neoplastic lesions mainly were breast fibroadenoma (25.86%) and pituitary adenoma (24.14%).The malignant neoplas-tic lesions mainly were adenocarcinoma (5.17%) and breast cancer (3.45%).Conclusions The spontaneous neoplastic lesions and their incidences reported in this paper provide another data of the spontaneous tumors of SPF Wistar rats and may provide some reference for relevant technical staffs .